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Image Search Results
Journal: bioRxiv
Article Title: Secreted phospholipase PLA2G12A-driven lysophospholipid signaling via lipolytic modification of extracellular vesicles facilitates pathogenic Th17 differentiation
doi: 10.1101/2024.10.27.620543
Figure Lengend Snippet: (A) Schematic representation of the procedure for IMQ-induced psoriasis. (B, C) Hindlimb swelling ( n = 8–18) (B) and clinical score (n = 4–9) (C) in Pla2g12a +/+ (+/+) and Pla2g12a -/- (–/–) mice over 42 days after immunization with type II collagen. (D) Representative photos of the hindlimbs of Pla2g12a +/+ and Pla2g12a -/- mice with or without CIA on day 42. (E) HE and TRAP staining of the knee joints from Pla2g12a +/+ and Pla2g12a -/- mice with (+) or without (–) CIA on day 42. Yellow arrowheads, bone erosion; white arrowheads, inflammatory cell infiltration. Scale bar, 100 µm. (F, G) μCT analysis of the hindlimb bone from Pla2g12a +/+ and Pla2g12a -/- mice with or without CIA on day 42. Yellow arrowheads, bone erosion; white arrowheads, cortical bone; asterisk, trabecular bone. Scale bar, 1 mm. Representative μCT images (F) and tissue mineral density of the cortical bone (G) are shown. (H) FACS of splenic Th17 cells in Pla2g12a +/+ and Pla2g12a -/- mice with or without CIA on day 42. Representative FACS profiles ( left ) and the proportion of Th17 cells ( right ) are shown. (I, J) ELISA of IL-17A (I) and anti-type II collagen (J) in sera of Pla2g12a +/+ and Pla2g12a -/- mice with or without CIA on day 42. Values are mean ± s.e.m.. Representative data of three (B, C, G) or two (H) experiments and combined results of two experiments (I, J) are shown. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test (G, H), Kruskal-Wallis with Dunn’s multiple comparisons test (I, J), and two-way repeated measures ANOVA with Sidak’s multiple comparisons test (B, C). *, P < 0.05; **, P < 0.01.
Article Snippet: Briefly, the
Techniques: Staining, Enzyme-linked Immunosorbent Assay